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KMID : 0613820110210111631
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Journal of Life Science
2011 Volume.21 No. 11 p.1631 ~ p.1635
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Enzymatic Synthesis of Polyphenol Glycosides by Amylosucrase
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Park Hyun-Su
Choi Kyoung-Hwa
Park Young-Don
Park Cheon-Seok
Cha Jae-Ho
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Abstract
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The capability of synthesizing polyphenol glycosides was examined using recombinant amylosucrase from the hyperthermophilic bacterium Deinococcus geothermalis. Based on the action mode of amylosucrase, sucrose and twenty-one polyphenols were used as a donor and acceptors respectively. The transglycosylation reaction by amylosucrase produced one or two major polyphenol glycosides depending on the type of polyphenols used. The synthesized polyphenol glycosides were detected by thin-layer chromatography. The structures of the newly synthesized polyphenol glycosides were predicted based on the transglycosylation mechanism of the enzyme. According to the acceptability of the polyphenols, the structural characteristics of polyphenol as an efficient acceptor were evaluated. The results indicate that amylosucrase is an efficient catalyst for the enzymatic synthesis of polyphenol glycosides, which have high potentials in food, cosmetics, and pharmaceutical industries.
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KEYWORD
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Acceptor reaction, amylosucrase, polyphenol glycosides, transglycosylation
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